Optimized process control in bioreactors

In a bioreactor it is important to keep the dissolved oxygen and pH at an optimal level at all times. Cell density and metabolites should also be monitored for a better insight into the process. Concentration of metabolites is typically measured off-line with time consuming techniques and therefore require sampling with the associated risks.

Our expertise in the field

Raman spectroscopy is an ideal solution for fast and non-destructive measurement. Our Raman solutions can monitor several parameters simultaneously with one single probe installed directly in-line. The complete upstream package enables real-time and optimized control of the process leading to reduced time between batches, limited risk of contamination and increased yield.

In-line glucose control

Glucose is the main energy source of cells during the upstream process. The concentration of glucose is a critical process parameter (CPP) because excessive glucose can lead to metabolic inefficiency and subsequent toxic lactate and glucose accumulation. Traditionally, a sample must be taken from the cultivation and analyzed in the lab to determine the amount of glucose that needs to be supplied to the bioreactor.

Our expertise in the field

Direct in-line monitoring of glucose concentration provides a better understanding and control of the fermentation or cell culture process. Continuous glucose measurements using Kaiser Raman analyzers not only offer important bioprocess information for better process understanding but also bring huge potential for optimization. For example, with Raman-based glucose control in a mammalian cell bioprocess, titer increase of up to 85% has been realized.

Protein monitoring in chromatography

As a pharmaceutical manufacturer, you strive for highest possible yield and purest product in your downstream process. Improving chromatography and the purification of your product mixture is a key aspect in achieving this goal. To achieve a maximum purification degree with minimum loss, you need precise and sensitive UV absorption with fast and reliable protein detection.

Our expertise in the field

With our UV absorption photometer, we bring the sensor and the measurement right into the process, by measuring the protein concentration in-line. For signal processing we use our well-known transmitter platform Liquiline. Using only one Liquiline CM44P transmitter, you can add pH and conductivity sensors, making it the perfect package for chromatography control.